ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of matrix metalloproteinase (MMP) in the transformation of subdural effusion into chronic subdural hematoma.</p><p><b>METHODS</b>The clinical data of 8 patients with subdural effusion that transformed into chronic subdural hematoma were collected and MMP-2 and MMP-9 levels were detected in the sudural effusion, chronic subdural hematoma and capsules of hematoma using gelatin-zymography.</p><p><b>RESULTS</b>MMP-2 and MMP-9 increased significantly in chronic subdural hematoma as compared with those in subdural effusion (P<0.01), and their levels were also significantly higher in the outer membrane than in the inner membrane of hematoma.</p><p><b>CONCLUSION</b>Subdural effusion is a risk factor for the occurrence of chronic subdural hematoma, in which process MMP plays a role as the promoting factor acting primarily in the outer membrane of the hematoma.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Chronic Disease , Hematoma, Subdural , Matrix Metalloproteinase 2 , Metabolism , Matrix Metalloproteinase 9 , Metabolism , Subdural EffusionABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of endogenous brain derived neurotrophic factor (BDNF) on GAP-43 expression in the anterior horn of the spinal cord in rats following sciatic nerve injury.</p><p><b>METHODS</b>BDNF antibody was injected intraperitoneally in rats with crushing injury of the sciatic nerve, and the control rats received normal saline only after sciatic nerve injury. At 7 and 14 days after the injection, the expression of GAP-43 in the anterior horn of the corresponding segments of the spinal cord was detected by Western blot and RT-PCR.</p><p><b>RESULTS</b>The expressions of GAP-43 protein and mRNA in the anterior horn of the spinal cord were significantly down-regulated in rats with BDNF antibody injection as compared with those in the control group (P<0.01).</p><p><b>CONCLUSION</b>Endogenous BDNF may regulate the expression of GAP-43 in the spinal cord anterior horn after sciatic nerve injury in rats.</p>
Subject(s)
Animals , Male , Rats , Anterior Horn Cells , Metabolism , Brain-Derived Neurotrophic Factor , Metabolism , Physiology , Down-Regulation , GAP-43 Protein , Genetics , Metabolism , RNA, Messenger , Genetics , Metabolism , Random Allocation , Rats, Sprague-Dawley , Sciatic Nerve , Wounds and Injuries , Spinal Cord , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To simulate the surgical approaches for intracranial aneurysms using three-dimensional CT angiography (3D-CTA) and assess the value of 3D-CTA in early microneurosurgery for ruptured intracranial aneurysms.</p><p><b>METHODS</b>Forty-eight patients with spontaneous subarachnoid hemorrhage due to ruptured intracranial aneurysm were confirmed by early operation. All the patients were classified according to Hunt-Hess, including 11 of grade I, 29 of grade II, and 8 of grade III. CTA was performed before the operation and surgical simulation was conducted. The preoperative findings on CTA and the intraoperative findings were compared and the clinical value of cerebral 3D-CTA was analyzed.</p><p><b>RESULTS</b>Pre-operative 3D-CTA clearly displayed the location, size and shape of the aneurysms, the axis direction of the aneurysm apex and the width of aneurysm neck. The spatial relation between the parent aneutysm artery, the aneurysm, the peripheral vessels and the bony structures were also demonstrated. These findings were basically consistent with the intraoperative findings. The Glasgow outcome score was 5 in 41 patients, 4 in 4 patients, 3 in 2 patients, and 2 in 1 patient upon discharge from the hospital.</p><p><b>CONCLUSIONS</b>Preoperative 3D-CTA examination can simulate the surgery for ruptured aneurysms to help improve the surgical success rate.</p>
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Aneurysm, Ruptured , Diagnostic Imaging , General Surgery , Cerebral Angiography , Methods , Computer Simulation , Imaging, Three-Dimensional , Intracranial Aneurysm , Diagnostic Imaging , General Surgery , Radiography, Interventional , Subarachnoid Hemorrhage , Diagnostic Imaging , General Surgery , Tomography, Spiral ComputedABSTRACT
OBJECTIVE@#To evaluate the use of endoscopic surgery for hypertensive cerebral hemorrhage.@*METHODS@#Sixteen patients with hyertensive intracerebral hematoma were evacuated with neuroendoscope. The surgical invasive markers, volume of remaining hematoma, and prognosis were compared with those of 19 comparable patients undergoing conventional craniotomy.@*RESULTS@#Complete evacuation of hematoma was achieved in 9 patients, and partial evacuation in 7. All patients were followed up for 6 months. According to GOS, the result was excellent in 6 patients, good in 6, fare in 2, poor and dead in one respectively. The volume of remaining hematoa and invasive markers significantly decreased (P 0.05).@*CONCLUSION@#Neuroendoscopic surgery for hypertensive intracerebral hematoma is characterized by mini-invasion, time-saving, and direct-vision, and is a new approach in this field.